Fig 1: Effect of expansion on human NP cell population doubling and phenotype in FCS alternatives. The effects of different media supplements on human NP cells from recently deceased organ donors (n = 4) were investigated by culturing cells in high glucose DMEM with three media supplement combinations (10% FCS, “No FCS” containing Albumax/proline/ITS-X, or “No FCS + FGF-2” containing Albumax/proline/ITS-X/FGF-2) and on either Geltrex-coated or uncoated tissue culture plastic until end of P0 for assessment of cell count (A) and qRT-PCR (B–E). All qRT-PCR was normalized to GAPDH and RNA45S5. Two-way ANOVA with Tukey's post hoc tests *P < 0.05.
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